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selenium colorimetric assay kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology selenium colorimetric assay kit
    Selenium Colorimetric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/selenium/Selenium+Colorimetric+Assay+Kit/pm42854606-59-0-8
    Average 94 stars, based on 6 article reviews
    selenium colorimetric assay kit - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    CCK-8 Assay:

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen-glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway.
    Article Snippet: .. 2.2 Experimental Materials HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen–glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway
    Article Snippet: .. HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen-glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway.
    Article Snippet: .. 2.2 Experimental Materials HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen–glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway
    Article Snippet: .. HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Iron Assay:

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen-glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway.
    Article Snippet: .. 2.2 Experimental Materials HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen–glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway
    Article Snippet: .. HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    GSH Assay:

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen-glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway.
    Article Snippet: .. 2.2 Experimental Materials HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen–glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway
    Article Snippet: .. HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Colorimetric Assay:

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen-glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway.
    Article Snippet: .. 2.2 Experimental Materials HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen–glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway
    Article Snippet: .. HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Article Title: Nano-selenium suppresses cuproptosis to restore mitophagic flux and alleviate deoxynivalenol-induced renal inflammation.
    Article Snippet: .. Selenium colorimetric assay kit (E-BC-K776-M) was ordered from Elabscience (Wuhan, China). .. H&E staining kit (C0105S), CCK-8 kit (C0037), Copper assay kit (S1075S), Succinate assay kit (S0529S), Mito-Tracker Green (C1048), Mito-SOX Red (S0061S), TMRE (C2001S), Lyso-Tracker Red (C1046), AdPlus-mCherry-GFP-LC3B(C3012), RIPA Lysis buffer (P0013B), 4% paraformaldehyde (P0099), BeyoTSA double label three-color multiplex fluorescence staining kit (P1349S) were all acquired from Beyotime Biotechnology (Shanghai, China).

    RNA Extraction:

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen-glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway.
    Article Snippet: .. 2.2 Experimental Materials HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen–glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway
    Article Snippet: .. HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen-glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway.
    Article Snippet: .. 2.2 Experimental Materials HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Article Title: Pramipexole alleviates ferroptosis in HT22 cells induced by oxygen–glucose deprivation/reoxygenation via the Nrf2/SLC7A11/GPX4 pathway
    Article Snippet: .. HRP conjugated Goat Anti-Rabbit IgG (GB23303) and HRP conjugated Goat Anti-Mouse IgG (GB23301) were purchased from Wuhan Servicebio Technology (Wuhan, China); Cell Counting Kit-8 (C0038), Enhanced BCA Protein Assay Kit (P10010) and Antifade Mounting Medium with DAPI (P0131) were obtained from Biyuntian Biotechnology (Shanghai, China); Pramipexole dihydrochloride powder (HY-17355), Erastin, JC-1 and H2DCFDA were provided by MedChemExpress (Monmouth Junction, NJ, USA); Tissue iron assay kit (A039-2-1) and GSH assay kit (A006-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); Glutathione peroxidase 4 (GPX4) colorimetric assay kit (E-BC-K883-M) was obtained from Elabscience Biotechnology (Wuhan, China); Ultra-fast single-column animal cell total RNA extraction kit (RC102-01), HiScript IV All-in-One Ultra RT SuperMix for qPCR (R433-01), SupRealQ Ultra Hunter SYBR qPCR Master Mix (Q713-02) were provided by Nanjing Vazyme Biotech (Nanjing, China); The primary antibodies used in this study were GPX4 (ab125066, Abcam, Cambridge, MA, UK) and X-CT (ab175186, Abcam, Cambridge, MA, UK); Nrf2 (16396-1-AP, Proteintech, Wuhan, China) and p53 (10442-1-AP, Proteintech, Wuhan, China). ..

    Activity Assay:

    Article Title: The Regulatory Role of Iron Transporter SLC39A13 in Liver Fibrosis
    Article Snippet: SOD activities in the liver of mice induced by CCl 4 were measured using the “Total Superoxide Dismutase Assay Kit ” (Beyotime, S0101S) and following the manufacturer's instructions. .. GPX4 activity in mouse liver tissue was quantified using a Glutathione Peroxidase 4 (GPX4) Activity Assay Kit (Elabscience, E‐BC‐K883‐M), according to the manufacturer's protocol. ..

    Article Title: The Regulatory Role of Iron Transporter SLC39A13 in Liver Fibrosis.
    Article Snippet: SOD activities in the liver of mice induced by CCl4 were measured using the “Total Superoxide Dismutase Assay Kit ” (Beyotime, S0101S) and following the manufacturer’s instructions. .. GPX4 activity in mouse liver tissue was quantified using a Glutathione Peroxidase 4 (GPX4) Activity Assay Kit (Elabscience, E-BC-K883-M), according to the manufacturer’s protocol. ..

    Article Title: Copper deprivation reprograms antioxidant defense to suppress ferroptosis via SLC7A11
    Article Snippet: Cell Total Iron Colorimetric Assay Kit , Elabscience , E-BC-K880-M. .. Glutathione Peroxidase 4 (GPX4) Activity Assay Kit , Elabscience , E-BC-K883-M. .. Ferroptosis Suppressor Protein-1 (FSP-1) Inhibitor Screening Kit , Elabscience , E-BC-D008.

    Article Title: Capsaicin ameliorates diabetic nephropathy by targeted activation of GPX4 and inhibition of pyroptosis
    Article Snippet: Simultaneously, the rats were anesthetized by intraperitoneal injection of 3% sodium pentobarbital (P3761, Sigma-Aldrich, China) at a dose of 40 mg/kg, and blood samples were collected via the abdominal aorta. .. Serum was separated for measurement of blood glucose (BC2495, Solarbio, China), blood urea nitrogen (S0574M, Beyotime, China), serum creatinine (S0291S, Beyotime, China), cystatin C (E-EL-R0304,Elabscience, China), kidney injury molecule 1 (KIM-1, E-OSEL-R0009, Elabscience, China), neutrophil gelatinase associated lipocalin (NGAL, PN758, Beyotime, China), glutathione peroxidase 4 (GPX4) activity (E-BC-K883-M, Elabscience, China), Glycated Hemoglobin A1c (HbA1c, ELK012, ELK Biotechnology, China) according to the manufacturers’ instructions. ..

    Article Title: Dietary advanced glycation products combined with chronic restraint stress induced anxiety-like and depression-like behaviors in male mice
    Article Snippet: Mouse interleukin-6 (IL-6) ELISA kit (cat no. EK206) and interferon-γ (IFN-γ) ELISA kit (cat no. EK280) were purchased from Multisciences Biotechnology (Hangzhou, China). .. Glutathione Peroxidase 4 (GPX4) activity assay kit (cat no. E-BC-K883-M) was purchased from Elabscience (Wuhan, China). .. Ferrostatin-1 (Fer-1) (cat no. GC10380), L-Kynurenine (cat no. GC12040) and 3-hydroxy-DL-Kynurenine (cat no. GC 45336) were purchased from GlpBio Technology (CA, USA).



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